Terminator

Part:BBa_B1008:Design

Designed by: Haiyao Huang   Group: Knight Lab   (2006-09-07)


Terminator (artificial, large,  %T~=70)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.



Source

modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair


References